GC/MS法检测油条用煎炸油中8种多环芳烃
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石家庄市疾病预防控制中心理化检验科,石家庄市化学毒物检测及风险预警技术创新中心, 河北 石家庄 050011

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董新凤 女 副主任技师 研究方向为食品风险监测及食品安全 Email: dxfeng2002@163.com

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R155

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河北省医学科学研究课题(20231706)


Determination of 8 polycyclic aromatic hydrocarbons in fried oil for churros using GC-MS
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Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang Technology Innovation Center for Chemical Poison Detection and Risk Early Warning, Hebei Shijiazhuang 050011, China

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    摘要:

    目的 建立一种固相萃取-气相色谱/质谱法检测油条用煎炸油中8种多环芳烃的方法。方法 样品经氢氧化钾乙醇溶液皂化后加正己烷提取,采用Eu多环芳烃柱净化,气相色谱/质谱仪分析测试。结果 8种多环芳烃在10~250 ng/mL范围内线性关系良好,相关系数R>0.997;检出限为0.10~0.20 μg/kg,定量限为0.35~0.70 μg/kg;3个浓度加标回收率为70.1%~110.7%,RSD为3.6%~10.9%。180份油条用煎炸油样品中8种多环芳烃有不同程度检出,90份未煎炸前油中检出苯并[α]芘4份,检出率最高的为?,检出32.2%;污染水平最高的为?,最高含量为12.7 μg/kg。90份多次煎炸后油中苯并[α]芘检出21份,?是最主要检出的多环芳烃,检出率为50.0%,污染水平最高为苯并[a]蒽(32.4 μg/kg)。结论 该方法数据可靠、方法灵敏度高,适用于油条用煎炸油中多环芳烃的测定。

    Abstract:

    Objective A method for the determination of PAH8 in fried oil for churros was developed by modified SPE-GC/MS.Methods The target analytes in samples were saponified by KOH/Ethanol, extracted with n-hexane and cleaned-up by solid phase extraction column, detected by GC-MS.Results The method shows satisfactory linearity (R>0.99) over the range assayed (10-250 ng/mL), and the limits of detection (LODs) range from 0.10-0.20 μg/kg, the limits of quantitation (LOQs) range from 0.35-0.70 μg/kg. The recoveries varied from 70.1% to 110.7% with relative standard deviation (RSDs) of 3.6% to 10.9% at spiked 3 levels. All PAH8 were detected in 180 samples. 4 benzo [α] pyrene was detected in 90 oil samples, the most detection rates of PAH8 was Chrysene (32.2%), and the highest mean concentration of PAH8 was 12.7 μg/kg for Chrysene. In 90 fried oil, 21 samples were detected benzo [α] pyrene , and Chrysene was the most detection rates of PAH8, accounting for 50.0%, the highest content was 32.4 μg/kg for benzo [a] anthracene.Conclusion The method has the characteristics of high sensitivity, low detection limit and good precision, and is suitable for rapid and accurate quantitative detection of PAH8 in Fried oil for churros.

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董新凤,冯佩,吴命君,赵伟,马玲. GC/MS法检测油条用煎炸油中8种多环芳烃[J].中国食品卫生杂志,2025,37(8):722-728.

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  • 收稿日期:2024-08-21
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