Objective To investigate the contamination of Listeria monocytogenes （L. monocytogenes） in commercially available fresh enoki mushrooms in Shijiazhuang City， and to analyze the genomic characterization of the strains.Methods A total of 49 samples of commercially available fresh enoki mushrooms were tested and characterized for L. monocytogenes， among which 18 strains of L. monocytogenes were isolated. All isolates were subjected to whole-genome sequencing to determine strain sequence type （ST）， clonal complex （CC）， lineage， and serogroup， using the BIGSdb-Lm database. The presence of virulence genes and drug resistance genes was predicted using the VFDB virulence gene database and the CARD database.Results Eighteen strains of L. monocytogenes were isolated from 49 samples， with a detection rate of 36.7%. Whole-genome sequencing results showed that all 18 isolated strains belonged to lineage Ⅱ. Among them， 11 strains belonged to ST8， CC8 and serogroup 1/2a， while 7 strains belonged to ST9， CC9， and serotype 1/2c. All isolated strains carried the LIPI-1 virulence island genes and some genes in LIPI-2. Strain Lm02 carried the lnu gene， which mediates resistance to lincosamide antibiotics； strain Lm05 carried the mprF gene， which mediates resistance to peptide antibiotics； 15 strains carried both the lnu and mprF genes； in addition， 7 strains carried the norB gene， which mediates resistance to fluoroquinolone antibiotics.Conclusion There is a risk of L. monocytogenes contamination in fresh enoki mushrooms sold in Shijiazhuang City. It is essential to strengthen the detection and control of L. monocytogenes to ensure consumer health.