Objective To explore the differences in phenotypic characteristics of Staphylococcus aureus from different sources on Baird-Parker agar medium （hereinafter referred to as BP medium） produced by four manufacturers， so as to provide data support for the optimization and improvement of the test method.Methods The selected Staphylococcus aureus （n=62） was confirmed， its growth characteristics on BP medium were analyzed， and the differences in colony occurrence time， phenotype and growth rate of four brands of BP medium and Staphylocophilia aureus chromogenic medium were compared.Results All 62 strains were confirmed to be Staphylococcus aureus by MOLDI-TOF and plasma coagulase assays. After 20-24 h of culture on BP medium， all strains （n=62） did not show the typical colony characteristics of a “clear band” on the periphery of the colony given in GB 4789.10—2016， and some strains had atypical colony phenotypes with weakened and missing precipitation rings and zona pellucida even after 44-48 h of culture. The growth rate of a few strains on different brands of BP medium was less than 0.7， and there were significant differences in the colony appearance time and growth rate of Staphylococcus aureus on different brands of BP medium （P<0.01）. The colonies of Staphylococcus aureus on the chromogenic plate were blue-green， with no obvious phenotypic difference， and the growth rate was greater than 0.7.Conclusion The growth characteristics of Staphylococcus aureus on BP medium are closely related to the culture time and medium quality， and the uncertainty caused by the use of a single selective medium should be considered in the revision of GB 4789.10—2016， and a second selective medium should be introduced into the methodology to enhance the reliability and rigor of the test results.