广东省2020—2022年O10:K4型副溶血性弧菌的病原学特征分析
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作者单位:

1.中国人民解放军勤联保障部队第九二一医院,湖南 长沙 410000;2.广东省疾病预防控制中心,广东省新发传染病应急检测技术研究与应用重点实验室,广东 广州 510080;3.南方医科大学公共卫生学院, 广东 广州 510080;4.广州中医药大学中药学院,广东 广州 510080

作者简介:

邹敏 女 硕士研究生 研究方向为营养与食品卫生 E-mail:1029847866@qq.com

通讯作者:

柯碧霞 女 主任技师 研究方向为预防医学 E-mail:kebixiagdcdc@163.com

中图分类号:

R155

基金项目:

国家科技重大专项(2018ZX10714002-003-006)


Pathogenic characteristics of Vibrio parahaemolyticus O10:K4 in Guangdong Province from 2020 to 2022
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Affiliation:

1.921 Hospital of the PLA Logistic Support Force, Hu’nan Changsha, 410000, China;2.Guangdong Provincial Key Laboratory of Emergency Detection Technology for Emerging Infectious Diseases, Guangdong Provincial Center for Disease Control and Prevention, Guangdong Guangzhou 510080, China;3.School of Public Health, Southern Medical University, Guangdong Guangzhou 510080, China;4.School of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangdong Guangzhou 510080, China

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    摘要:

    目的 了解广东省2020-2022年O10:K4型副溶血性弧菌的耐药特性、分子特征及其流行情况,为副溶血性弧菌感染的防治提供科学依据。方法 对2020—2022年广东省分离自感染患者临床标本的副溶血性弧菌菌株进行血清学鉴定、药敏实验、多位点序列(MLST)分型和全基因组测序,同时利用微生物基因注释系统对测序结果开展耐药基因与毒力因子注释。结果 290株副溶血性弧菌可分为24种血清型,其中O10:K4占31.38%(91/290)。91株O10:K4型副溶血性弧菌药敏试验显示,所有菌株均对氨苄西林中等耐药,对其他药物敏感。在290株副溶血性弧菌菌株中选取75株开展全基因组测序,经MLST分型和系统发育进化关系分析发现,75株菌株中,O10:K4型副溶血性弧菌共48株,其中ST3型共47株,为主要型别(97.92%,47/48),且与O3:K6菌株遗传进化距离最近,还出现了4种新型副溶血性弧菌基因序列型别:ST3485、ST3486、ST3488和ST3494。O10:K4型副溶血性弧菌具有大流行菌株的独特遗传标志(toxRS/new+、orf8+、tdh+和trh-)且携带多种毒力基因,毒力基因分布与O4:KUT和O3:K6血清型相似。结论 2020—2022年广东省副溶血性弧菌以O10:K4为优势血清型,与O3:K6流行克隆群具有类似分子特征且对多种抗菌药物敏感。副溶血性弧菌基因序列呈现高度多样性且具有明确的系统发育分支,以ST3为本地优势克隆群。

    Abstract:

    Objective To investigate the drug resistance, molecular characteristics and prevalence of Vibrio parahaemolyticus O10:K4 in Guangdong Province from 2020 to 2022, and to provide evidence for the prevention and control of Vibrio parahaemolyticus infection.Methods Serological identification, drug susceptibility test, MLST typing and whole genome sequencing were performed for Vibrio parahemolyticus strains isolated from clinical samples of infected patients in Guangdong Province from 2020 to 2022. At the same time, the microbial gene annotation system was used to annotate the drug resistance genes and virulence factors.Results The 290 strains of Vibrio parahaemolyticus could be divided into 24 serotypes, of which O10:K4 accounted for 31.38% (91/290). Drug susceptibility test of 91 strains of Vibrio parahaemolyticus O10:K4 showed that all strains were resistant to ampicillin and sensitive to other drugs. A total of 75 of 290 strains of Vibrio parahaemolyticus were selected for whole genome sequencing. The MLST typing and phylogenetic evolutionary relationship analysis showed that ST3 was the main sequence type of Vibrio parahaemolyticus O10:K4 (97.92%, 47/48) and was the closest genetic evolution to O3:K6. In addition, four novel Vibrio parahemolyticus sequence types were also identified in this study: ST3485, ST3486, ST3488 and ST3494. Vibrio parahaemolyticus O10:K4 has unique genetic markers of pandemic strains (toxRS/new+, orf8+, tdh+, and trh-) and carries multiple virulence genes, and the distribution of virulence genes is similar to that of serotypes O4:KUT and O3:K6.Conclusion The dominant serotype of Vibrio parahaemolyticus in Guangdong Province from 2020 to 2022 is O10:K4, which has similar molecular characteristics to O3:K6 and is sensitive to multiple antibiotics. Vibrio parahemolyticus sequences showed high diversity and clear phylogenetic clades, with ST3 as the local dominant clonal group.

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邹敏,何冬梅,欧阳方竹,王博俊,黄玉梅,陈启方,陈乐妍,柯昌文,柯碧霞.广东省2020—2022年O10:K4型副溶血性弧菌的病原学特征分析[J].中国食品卫生杂志,2025,37(2):124-130.

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  • 收稿日期:2024-08-08
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