Objective To develop a method for the determination of 12 organophosphate esters （OPEs） fire retardants in honey by ultra-high-performance liquid chromatography-high-resolution mass spectrometry.Methods The samples were enriched， extracted and cleaned-up by a sugar-induced acetonitrile-water liquid-liquid extraction system， eluted with a gradient of methanol-formic acid-water， and then separated on a HSS T3 chromatographic column （2.1 mm×100 mm， 1.8 μm）. The analytes were determined by high-resolution mass spectrometry in the targeted single-ion monitoring mode， with the quantification by isotope internal standard method.Results The linearity of the 12 OPEs， with R2>0.99， was observed from 0.2 to 20 μg/L， while the range of the LODs and the LOQs were 0.05-0.3 μg/kg and 0.1-0.9 μg/kg， respectively. Recoveries of the 12 OPEs at three different spiked levels ranged from 86.2% to 119.5%， with RSDs ranging from 1.24% to 8.16%. Analysis of 16 honey samples revealed 100% detection of TPhP at concentrations ranging from 1.7 to 11.8 μg/kg and 50% detection of TEP at levels from LOD to 1.5 μg/kg.Conclusion The method is convenient， reproducible， sensitive and suitable for the trace determination of OPEs in honey.