Objective To understand the residue levels of quinolone antibiotics residues in commonly marketed freshwater fish and shrimp from Shandong Province and risk assessment of dietary exposure.Methods From 2021 to 2023， a total of 480 freshwater fish and shrimp were collected from 16 prefecture-level cities in Shandong Province， the residues of six quinolone antibiotics were detected using solid-phase extraction coupled with ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）， the dietary exposure was calculated to assess the risk of antibiotics exposure in freshwater fish and shrimp.Results The total detection rate of quinolone antibiotics in marketed freshwater fish and shrimp in Shandong Province from 2021 to 2023 was 36.0%（173/480）， with an exceedance rate of 3.54%（17/480）. Among the six antibiotics， enrofloxacin was detected in the largest number of samples， with a detection rate of 34.8% （167/480）； followed by ciprofloxacin， with a detection rate of 7.08% （34/480）； ofloxacin was detected in three samples； lomefloxacin was detected in two samples； pefloxacin and norfloxacin were not detected in any of the 480 samples. The detection rate of fish was higher than that of shrimp， and the difference was statistically significant （P<0.05）. The average and maximum exposures of all age groups were less than the acceptable daily intake.Conclusion There are quinolone antibiotics residues in freshwater fish and shrimp in Shandong Province， and the exposure risk is low，but the exposure risk of children is higher than that of adults， and continuous monitoring and continuous monitoring and supervision should be strengthened.