Objective To provide the technical support for precise prevention and control of mushroom poisoning， the species and distribution of wild mushrooms in areas with high incidence of mushroom poisoning in Sichuan Province were understood.Methods Random inspection was used to collect samples in some areas of Mianyang， Yibin， and Ganzi Tibetan Autonomous Prefecture. Molecular biology methods combined with morphological characteristics were used. The ITS sequence of the samples was amplified and identified.Results A total of 364 samples were collected， which were identified as belonging to 39 families， 81 genera， and 215 species. The dominant families included Russulaceae， Agaricaceae， Omphalotaceae， Entolomataceae and Amanitaceae， accounting for 56.74% of the total of species. The dominant genera wre Russula， Lactarius， Amanita etc. accounting for 51.87% of the total species. There were 19 types of poisonous mushrooms， accounting for 8.84% of the total number. The clinical types were mainly acute liver damage， neuropsychiatric， and gastroenteritis. There were differences in the dominant families and genera in different regions.Conclusion Some areas of Sichuan Province have abundant wild mushroom resources. This study provides a foundation for a deeper understanding of the status of wild mushroom resources in Sichuan Province and the prevention and control of wild mushroom poisoning in the future.