2019—2021年兰州市腹泻患者肠炎沙门菌耐药特征及分子分型研究
作者:
作者单位:

兰州市疾病预防控制中心,甘肃 兰州 730030

作者简介:

毛雪 女 主管检验师 研究方向为微生物检验 E-mail:516030224@qq.com

通讯作者:

中图分类号:

R155

基金项目:

兰州市卫生健康科技发展项目(2021025)


The study of drug resistance characteristics and molecular typing of Salmonella enteritidis in Lanzhou City from 2019 to 2021
Author:
Affiliation:

Lanzhou Center for Disease Control and Prevention, Gansu Lanzhou 730030, China

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    摘要:

    目的 了解兰州市肠炎沙门菌的耐药谱及耐药特点,建立兰州市沙门菌基因型数据库,为有效预防沙门菌的感染及治疗提供科学参考。方法 对2019—2021年从兰州市食源性主动监测中分离到的94株沙门菌进行血清分型、生化鉴定;利用微量肉汤稀释法进行药物敏感性试验,利用脉冲场凝胶电泳(PFGE)检测41株肠炎沙门菌的指纹图谱;使用BioNumerics软件对41株肠炎沙门菌PFGE指纹图谱进行聚类分析。结果 94株沙门菌分属为20个血清型,其中肠炎沙门菌为优势血清群,占43.62%(41/94)。41株肠炎沙门菌对喹诺酮类萘啶酸的耐药率为100.00%,对青霉素类氨苄西林耐药率高达80.49%(33/41),对头孢唑林的耐药率为39.02%(16/41),对氨苄西林/舒巴坦、四环素的耐药率均为31.71%(13/41),但对亚胺培南、头孢西丁的耐药率为0.00%;其中26株菌(63.41%,26/41)出现多重耐药,共产生18种耐药谱。41株肠炎沙门菌通过Xba Ⅰ酶切共获得19种带型,各带型之间的相似度为74.20%~100.00%,各带型所包含的菌株数不同,同一年份菌株间的同源性较高并且有100.00%相同带型。结论 兰州市沙门菌血清型呈多样性,其优势血清群肠炎沙门菌高耐药和多重耐药严重,PFGE带型复杂多样,呈现出一定的遗传多样性。

    Abstract:

    Objective To provide scientific value for effective prevention of Salmonella infection and treatment, the drug resistance spectrum and drug resistance characteristics of Salmonella enteritidis in Lanzhou was exploreed and a database of some genotypes of Salmonella was established.Methods A total of 94 strains of Salmonella were isolated from foodborne active surveillance in Lanzhou from 2019 to 2021 by serotyping and biochemical analysis. The drug resistance of 41 strains of Salmonella enteritidis was detected by drug susceptibility testing and pulsed field gel electrophoresis. BioNumerics (Version 7.6) software was used to cluster the PFGE profiles of 41 strains of Salmonella enteritidis.Results A total of 94 strains of Salmonella could be classified into 20 serotypes, with Salmonella enteritidis as the dominant serogroup, accounting for 43.62% (41/94). 41 strains of Salmonella enteritidis were generally resistant to quinolone nalidixic acid bacteria (100.00%), followed by up to 80.49%(33/41) resistance to penicillin ampicillin, the resistance rate to cefazolin was 39.02%(16/41), and the resistance rates to ampicillin/sulbactam and tetracycline were 31.71%(13/41). But the drug resistance rates were all 0.00% to imipenem and cefoxitin. Twenty-six (63.41%) of the strains showed multi-drug resistance, resulting in eighteen multi-drug resistance profiles. Lastly, A total of 19 bands were obtained by Xba Ⅰ digestion of 41 strains of Salmonella enteritidis, and the similarity between the bands was 74.20%~100.00%. The number of strains contained in each band type was different, and the homology between strains of the same year was highly and they have 100.00% identical bands.Conclusion Salmonella serotypes in Lanzhou are diverse and PFGE band types are complex, and its dominant serogroup Salmonella enteritidis is highly resistant and multi-drug resistant seriously.

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毛雪,张连梅,王宇红,王艳,程徽,张继军,邸萌婕,刘璐.2019—2021年兰州市腹泻患者肠炎沙门菌耐药特征及分子分型研究[J].中国食品卫生杂志,2024,36(7):805-811.

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  • 收稿日期:2023-08-01
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  • 在线发布日期: 2024-10-23
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