2017—2021年温州市腹泻患者沙门菌血清学和耐药谱及分子分型特征分析
作者:
作者单位:

1.浙江中医药大学,浙江 杭州 310053;2.温州市疾病预防控制中心,浙江 温州 325000

作者简介:

林谦阁 女 检验技师 研究方向为微生物检验 E-mail:linqiange0709@163.com

通讯作者:

李毅 男 主任技师 研究方向为微生物检验 E-mail:zjwzliyi@126.com
孙桂芹 女 副教授 研究方向为微生物检验 E-mail:sunguiqin2001@163.com李毅和孙桂芹为共同通信作者

中图分类号:

R155

基金项目:


Serological, drug resistance spectrum and molecular typing analysis of Salmonella in patients with foodborne diarrhea in Wenzhou City from 2017 to 2021
Author:
Affiliation:

1.Zhejiang Chinese Medical University, Zhejiang Hangzhou 310053, China;2.Wenzhou Center for Disease Control and Prevention, Zhejiang Wenzhou 325000, China

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    摘要:

    目的 探究温州市食源性腹泻患者沙门菌的血清学、耐药性和分子分型特征,为食源性疾病的预防控制和临床治疗提供科学依据。方法 对从温州市2017—2021年食源性腹泻病例中分离出的567株沙门菌进行病患信息整理以及血清分型,使用微量肉汤稀释法对15种抗菌药物进行敏感性试验,运用脉冲场凝胶电泳(PFGE)技术对菌株进行分子分型。结果 567株沙门菌腹泻患者含男性326人,女性241人,分别占57.5%和42.5%,多为6岁以下的儿童,共分离出119株,占21.0%;其次为60岁以上的老年人,共分离出111株沙门菌,占19.6%。567株沙门菌共分为50种血清型,其中位居前两位血清型是鼠伤寒沙门菌(33.5%,190/567)和肠炎沙门菌(23.6%,134/567)。567株沙门菌对氨苄西林耐药率最高,为73.5%(417/567),其次四环素为63.3%(359/567);鼠伤寒沙门菌对四环素的耐药率最高为84.2%(160/190),而肠炎沙门菌对萘啶酸的耐药率最高为95.5%(128/134)。多重耐药率为62.6%,共产生178种耐药谱,其中最多的耐药谱为AMP-AMS-CFZ-CT-NAL。201株鼠伤寒沙门菌及其单相变种产生70种带型,带型差异度较大,相似度在1.5%~100%之间;127株肠炎沙门菌产生32种带型,相似度在35.8%~100%之间。50株伦敦沙门菌共产生24种带型,相似度在40.0%~100%之间。结论 温州市食源性沙门菌呈现广泛的血清型分布,并存在不同程度的耐药性及多重耐药趋势。为了有效防控沙门菌引起的食源性疾病,应加强对食源性腹泻患者沙门菌的监测,及时了解温州市血清型分布趋势和耐药特点,并采取必要的预防措施。

    Abstract:

    Objective To provide scientific basis for the prevention and control of foodborne diseases and clinical treatment, the serological, drug resistance and molecular typing characteristics of Salmonella isolated from patients with foodborne diarrhea in Wenzhou City was investigated.Methods A total of 567 Salmonella strains isolated from foodborne diarrhea cases in Wenzhou City from 2017 to 2021 were sorted out and serotyped. The susceptibility tests to 15 antimicrobial agents were performed using the broth microdilution method. Pulsed field gel electrophoresis (PFGE) was used for molecular typing.Results Among the 567 patients of Salmonella, 326 were male and 241 were female, accounting for 57.5% and 42.5%, respectively. Most of them were children under 6 years old, and 119 strains were isolated, accounting for 21.0%. Next was the elderly over 60 years old, 111 strains of Salmonella were isolated, accounting for 19.6%. The 567 isolates of Salmonella were divided into 50 serotypes, among which the top two serotypes were Salmonella typhimurium (33.5%, 190/567) and Salmonella enteritidis (23.6%, 134/567). The results showed that the resistance rate to ampicillin was the highest (73.5%, 417/567). It was followed by tetracycline (63.3%, 359/567). Salmonella typhimurium had the highest resistance to tetracycline (84.2%, 160/190), and Salmonella enteritidis had the highest resistance to nalidixic acid (95.5%, 128/134) among 15 antibiotics. Among 567 strains of Salmonella, 178 kinds of drug resistance spectrum were found, the rate of multiple drug resistance was 62.6%and the highest proportion was AMP-AMS-CFZ-CT-NAL. The 201 strains of Salmonella typhimurium and the monophasic varieties of Salmonella typhimurium produced 70 band types. The band types varied greatly and the similarity was between 1.5% and 100%. One hundred and twenty-seven strains of Salmonella enteritidis obtained a total of 32 band types with the similarity between 35.8% and 100%. Fifty strains of Salmonella london obtained a total of 24 band types by PFGE, and the similarity was between 40.0% and 100%.Conclusion Foodborne Salmonella strains isolated in Wenzhou displayed diverse serotypes, varying degrees of drug resistance, and multi-drug resistance. To effectively prevent and control foodborne diseases caused by Salmonella, enhancing monitoring of Salmonella among foodborne diarrhea patients was essential. Timely understanding of Salmonella serotype distribution trends and drug resistance patterns in the region, followed by appropriate measures, was imperative.

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林谦阁,章乐怡,胡玉琴,孙桂芹,李毅.2017—2021年温州市腹泻患者沙门菌血清学和耐药谱及分子分型特征分析[J].中国食品卫生杂志,2024,36(7):788-796.

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  • 收稿日期:2023-07-19
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  • 在线发布日期: 2024-10-23
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