2020年绍兴市145株分离自食源性腹泻患者和生禽肉的沙门菌表型及基因组特征分析
作者:
作者单位:

绍兴市疾病预防控制中心,浙江 绍兴 312071

作者简介:

张琴超 女 主管技师 研究方向为食源性疾病主动监测 E-mail:984114654@qq.com

通讯作者:

何琴芬 女 主管技师 研究方向为食品安全风险监测 E-mail:185449314@qq.com

中图分类号:

R155

基金项目:

浙江省公共卫生应急检测关键技术重点实验室开放基金(2020-02);绍兴市科技计划项目基础公益类(2023A14019)


Phenotypic and genomic characterization of 145 Salmonella strains from foodborne patients and raw poultry in Shaoxing City in 2020
Author:
Affiliation:

Shaoxing Center for Disease Control and Prevention, Zhejiang Shaoxing 312071, China

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    摘要:

    目的 监测绍兴市临床分离株和食品分离株沙门菌的分子分型特征和耐药情况,分析部分临床分离株的基因组特征。方法 采用玻片凝集法鉴定血清型别,脉冲场凝胶电泳(PFGE)技术应用于分子分型,微量肉汤稀释法检测药物耐药性。开展全基因组测序,使用BioNumerics V7.6软件进行全基因组单核苷酸多态性(wgSNP)分型,采用Pubmlst确定ST型别,使用ResFinder 4.1抗性基因数据库和VFDB毒力基因数据库预测耐药基因和毒力基因。结果 135株临床分离株和10株食品分离株沙门菌共检出24种血清型、109种PFGE指纹图谱、80种耐药表型,其中优势血清为鼠伤寒沙门菌、肠炎沙门菌、伦敦沙门菌,其中食品分离株以伦敦沙门菌和乙型副伤寒沙门菌为主;主要耐氨苄西林、四环素、链霉素,且多重耐药率达73.79%(107/145),其中食品分离株以耐氨苄西林、头孢唑啉、四环素和萘啶酸抗生素为主。30株临床分离株沙门菌有11个ST型别,ST11型的4株肠炎沙门菌属于同一进化枝,具有较近的遗传进化关系。共预测31种耐药基因,其中氨基糖苷类耐药基因aac(6')-Iaa携带率达100%(30/30),其次为β-内酰胺类耐药基因blaTEM-1B(70%,21/30);预测出224种毒力基因,其中107种携带率达100%(30/30)。结论 绍兴市沙门菌血清型、PFGE图谱、耐药表型种类多样,多重耐药率较高,且选取的生禽肉不是导致绍兴市沙门菌感染的直接来源;部分临床分离株的耐药基因和毒力基因携带率较高。

    Abstract:

    Objective To monitor the molecular epidemiology and antimicrobial resistance pattern of Salmonella isolates recovered from the clinical and the food sources in Shaoxing City, the genomic characterization of some clinical strains were analyzed.Methods Slide agglutination method was used to determine the serovars. PFGE method was applied to study molecular classification. Broth microdilution method was performed to carry out drug resistance testing. Whole genome data were sequenced. Whole genome single nucleotide polymorphism (wgSNP) classification was performed by BioNumerics V7.6 software. The sequence types (ST), drug resistant genes and virulence genes were predicted with Pubmlst, ResFinder 4.1 and VFDB software, respectively.Results Twenty-four serotypes, 109 different PFGE fingerprints, 80 resistant patterns were detected in 135 clinical and the 10 food isolates of Salmonella. The predominant serovars were Salmonella typhimuriumSalmonella enteritidis and Salmonella london. The main serotypes in food were Salmonella london and Salmonella paratyphi B. Salmonella had high resistance to ampicillin, tetracycline and streptomycin. The multiple drug resistance rate reached 73.79% (107/145). The main drug resistance in food were ampicillin, cefazolin, tetracycline and nalidixic acid. A total of 11 diverse ST types were identified from the 30 clinical strains. Phylogenetic wgSNP comparisons demonstrated that four Salmonella enteritidis from ST11 types were genetically similar and clustered together. A total of 31 different antimicrobial resistance genes among these isolates were identified. The most common antimicrobial resistance genes were the aminoglycosides resistance gene aac(6')-Iaa (100%, 30/30), the β-lactams resistance gene blaTEM-1B (70%, 21/30) and so on. A total of 224 virulence genes were also identified, and 107 of these existed among all isolates.Conclusion Salmonella in Shaoxing had a variety of serotypes and PFGE fingerprints, a complex resistant patterns, a high proportion of multiple drug resistance. The findings showed that selected raw poultry was not a direct source of local Salmonella infection. Part of the clinical strains had a high carrying rate of antimicrobial resistance genes and virulence genes.

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张琴超,周梁康,陈吉铭,唐诗洁,何琴芬.2020年绍兴市145株分离自食源性腹泻患者和生禽肉的沙门菌表型及基因组特征分析[J].中国食品卫生杂志,2024,36(7):774-781.

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  • 收稿日期:2023-03-07
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  • 在线发布日期: 2024-10-23
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