High content analysis combines automated microscopy and quantitative image analysis to visualize in vitro models for multi-parameter analysis， which has the advantages of high efficiency， rapidity and multi-targeting， and it has been applied in the field of in vitro toxicology research. Food toxicology， as a branch of toxicology， plays an important role in safeguarding food safety and human health. Traditional experiments can hardly meet the current rapidly increasing demand for safety evaluation of various food-related risk substances， for which high content analysis provides an efficient technical approach. Recent applications of high content analysis in the field of food toxicology would be reviewed in this article， in order to provide reference for food safety assessment methods and technological innovation.