2020—2022年泰州市预包装糕点中97株呕吐型蜡样芽胞杆菌的分析
作者:
作者单位:

泰州市食品检验院,江苏省市场监管重点实验室-特医食品风险识别及关键分析技术,江苏 泰州 225300

作者简介:

王萍 女 高级工程师 研究方向为食品微生物检测 E-mail:653539465@qq.com

通讯作者:

张占林 女 工程师 研究方向为食品微生物检测 E-mail:921138200@qq.com

中图分类号:

R155

基金项目:

江苏省市场监督管理局科技计划(KJ204140)


Analysis of 97 emetic Bacillus cereus strains in prepackaged pastries in Taizhou City from 2020 to 2022
Author:
Affiliation:

Taizhou Institute for Food Inspection, Jiangsu Provincial Key Laboratory of Market Supervision-Risk Identification and Key Analysis Technology of Food for Special Medical Purposes, Jiangsu Taizhou 225300, China

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    摘要:

    目的 了解泰州市预包装糕点中呕吐型蜡样芽胞杆菌腹泻型毒力基因、耐药性及分子分型情况。方法 利用聚合酶链反应(PCR)方法对泰州市预包装糕点中97株蜡样芽胞杆菌分离株进行呕吐型菌株的鉴别,并检测呕吐型菌株是否携带致腹泻的毒力基因,用纸片扩散法对呕吐型菌株进行19种抗生素的敏感性测试,用多位点序列分型技术进行分子分型。结果 97株蜡样芽胞杆菌中共检出5株呕吐型蜡样芽胞杆菌,分为ST 26和ST 1186两个型别;非溶血性肠毒素基因nhe、肠毒素基因entFMbceT是5株菌株的主要腹泻型毒力基因;5株菌株对青霉素、氨苄西林、头孢噻肟完全耐药,对复方新诺明、利福平、头孢唑啉的耐药率分别为80%(4/5)、40%(2/5)、20%(1/5),对阿莫西林、克林霉素、头孢西丁的敏感率分别为40%(2/5)、80%(4/5)、80%(4/5),对其他抗生素则完全敏感。结论 本研究显示泰州市预包装糕点中呕吐型蜡样芽胞杆菌的检出率为5.2%(5/97),以ST 26型为主要型别,携带多种腹泻型毒力基因。

    Abstract:

    Objective To understand the diarrhea-type virulence genes, the antimicrobial resistance and the molecular typing of emetic Bacillus cereus from the pre-packaged pastries in Taizhou City.Methods The Polymerase Chain Reaction (PCR) method was used to identify the vomiting strains of 97 Bacillus cereus strains which were isolated from pre-packaged pastries in Taizhou and detecte whether the vomiting strains carry diarrhea virulence genes. The sensitivity of the vomiting strains to 19 antibiotics was tested by disk diffusion method, and the molecular typing was carried out by multilocus sequence typing technique.Results The results showed that five emetic Bacillus cereus stains were detected of 97 Bacillus cereus strains and divided into ST 26 and ST 1186 two types. Non-hemolytic enterotoxin gene nhe, enterotoxin genes entFM and bceT were the main diarrhea-type virulence genes of the five strains. The five strains were completely resistant to penicillin, ampicillin and cefotaxime. The resistance rates to cotrimoxazole, rifampicin and cefazolin were 80%(4/5), 40%(2/5) and 20%(1/5), respectively. The sensitivity rates of amoxicillin, clindamycin and cefoxitin were 40%(2/5), 80%(4/5) and 80%(4/5), respectively, and they were completely sensitive to other antibiotics.Conclusion This study showed that the detection rate of vomiting Bacillus cereus in pre-packaged pastries in Taizhou was 5.2%(5/97), ST 26 was the main type, there were a variety of diarrhea-type virulence genes in the five vomiting Bacillus cereus strains.

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王萍,陈琳,乔勇升,胡慧,董贵军,张占林.2020—2022年泰州市预包装糕点中97株呕吐型蜡样芽胞杆菌的分析[J].中国食品卫生杂志,2024,36(6):653-658.

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  • 收稿日期:2023-07-24
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  • 在线发布日期: 2024-09-18
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