Objective To lay the foundation of quickly and effectively response to such epidemics for laboratories， the detection of the real-time PCR and culture test results of stool and anal swab samples from 3 outbreaks caused by Campylobacter was compared.Methods Biological samples from the 3 outbreaks of Campylobacter were collected. Bacterial culture was performed on the samples using filter culture method. DNA was extracted from the original samples， 24 h enriched samples and 48 h enriched samples. Kappa test was used to analyze the results of real-time PCR and culture method.Results The real-time PCR detection sensitivity of original samples， 24 h enriched samples and 48 h enriched samples were 90.91%， 97.22% and 100%. And the detection specificity were 75.00%， 84.00%和78.95%， respectively. Kappa value for the consistency analysis in culture method with real-time PCR based on original samples， 24 h enriched culture samples and 48 h enriched samples were 0.643， 0.813 and 0.785， respectively.Conclusion The combination of real-time PCR detection and culture detection is an effective laboratory testing method for the respond of Campylobacter outbreaks.