Vibrio parahaemolyticus （VP） is one of the most common pathogens that cause food poisoning in coastal areas of China. In fact， the food safety incidents of VP have been increasing on a yearly basis. The current national standard method for quantitatively detecting VP in food is the most probable number method. This method is time-consuming and tedious， and cannot be used to achieve rapid quantitative detection. Owing to modern biotechnology， new quantitative methods have been generated. In fact， a series of rapid and sensitive methods have been developed to quantify VP. This review seeks to discuss recent research and application progress of rapid methods for quantifying VP using biotechnology methods， and highlight the characteristics of each rapid method to provide a theoretical reference and development direction for the rapid and accurate quantitation of VP in food.