Objective The study investigated the evolutionary associations of drug resistance in main serotype Salmonella enteritidis （S. enteritidis）in Jiamusi City， to provide a scientific basis for clinical treatment.Methods S. enteritidis was isolated from food and patients with diarrhea. Serological typing was performed. Resistance against 16 antibiotics was investigated using the microbroth dilution method. Genes associated with antimicrobial resistance were analyzed by PCR， and the evolutionary relationships between strains were analyzed by pulsed-field gel electrophoresis （PFGE）.Results Of the 108 Salmonella strains identified， 55 （50.93%） were found to be S. enteritidis. Forty-nine （89.09%） of these strains showed antimicrobial resistance， with 33 strains （67.35%， 33/49） found to be resistant to multiple antibiotics. The highest resistance was against nalidixic acid and ampicillin， with 81.82% and 60.00% resistance， respectively. Homologies between 37% and 100% were observed among the 55 S. enteritidis strains.Conclusion High levels of multi-antimicrobial resistance were found in S. enteritidis in Jiamusi. A close relationship was found between， pathogen strain and drug resistance. S. enteritidis in the diarrhea of infected patients can cross-infect food. As S. enteritidis shows significant drug resistance in this region， it is of great importance to develop targeted diagnostic and treatment programs， as well as prevention and control measures， to ensure the safety and maintenance of public health.