Objective To investigate the infection of stage Ⅲ Anisakis spp. larvae in marine fish in five areas of Fujian Province.Methods Marine fish of different species were purchased from seafood markets from October 2021 to May 2022 and were directly dissected. The internal organs and muscles were artificially digested to identify stage Ⅲ Anisakis larvae， and the species were identified via light microscopy. The infection of Anisakis larvae was then analyzed by geographic area and species.Results A total of 133 marine fish from 18 species were dissected， with 68 fishes of 12 species being infected with Anisakis larvae； 2 214 larvae were detected. The detection rate， total infection rate and infection degree of Anisakis larvae were 66.7% （12/18）， 51.1% （68/133） and 32.6 larvae/tail （2 214/68）， respectively. Among the fish species， the infection rate of Trichiurus lepturus was 416 larvae/tail. The marine fish species in which Anisakis larvae were not detected were Acanthopagrus latus， Hypomesus olidus， Oriental grouper， cuttlefish， whitefish， and cream fish. According to regional distribution， the Changle area had the highest infection rate （66.7%）， while the highest detection rate of different marine fish varieties among the different areas was in Xiangcheng City （71.4%）.Conclusion The infection of stage Ⅲ Anisakis larvae in marine fish in the five areas of Fujian Province was serious. The distribution density of Anisakis larvae in marine fish and the detection rate of Anisakis larvae in each area were high. Hence， residents in these five areas of the Fujian Province have a high risk of infection.