一起疑似由蜡样芽胞杆菌引起的食源性疾病事件的分子溯源研究
作者:
作者单位:

1.上海市疾病预防控制中心,上海 200336;2.上海市长宁区疾病预防控制中心,上海 200051;3.上海市闵行区疾病预防控制中心,上海 201101

作者简介:

陈雯杰 女 主管医师 研究方向为食源性病原菌检测 E-mail: chenwenjie@scdc.sh.cn
陆冬磊 男 主任医师 研究方向为食源性疾病事件流行病学调查 E-mail: ludonglei@scdc.sh.cn

通讯作者:

张红芝 女 主任技师 研究方向为食源性致病菌分子溯源 E-mail: zhanghongzhi@scdc.sh.cn

中图分类号:

R155

基金项目:


Molecular tracing of a suspected foodborne disease event caused by Bacillus cereus
Author:
Affiliation:

1.Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China;2.Changning District Center for Disease Control & Prevention, Shanghai 200051, China;3.Minhang District Center for Disease Control & Prevention, Shanghai 201101, China

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    摘要:

    目的 对一起食源性疾病事件中食品分离蜡样芽胞杆菌(B. cereus)进行溯源分析,为确定污染源、切断传播途径提供技术支撑。方法 本研究建立脉冲场凝胶电泳(PFGE)方法,对12株B. cereus进行分子分型,同时对10株B. cereus进行全基因组测序(WGS),利用BioNumerics软件对测序数据进行拼接组装、多位点序列分型(MLST)、毒力基因、核苷酸多态性(SNP)分析。结果 本起食源性疾病事件中分离自不同食品的12株B. cereus的PFGE分型显示为8种带型,其中3株ST1435型 B. cereus带型相同,且SNP分析显示这3株B. cereus只有3个碱基差异;2株ST24型B. cereus 的PFGE带型完全相同,且SNP分析显示只有1个碱基差异,提示3株ST1435和2株ST24菌株分别为克隆株。2株ST24型B. cereus携带溶血性肠毒素hlbA、hlbC和hlbD,另有4株B. cereus携带腹泻毒力基因(hlbAhlbChlbD结论 B. cereus引起的食源性疾病事件比较复杂,污染源也比较复杂,因此加强原辅料监测、从业人员的卫生监测、环境和设备及环节的清洗消毒,对预防控制由其引起的食源性疾病事件非常重要。

    Abstract:

    Objective To trace Bacillus cereusB. cereus) from foodborne disease outbreaks toidentify pathogens and cut off transmission.Methods Pulsed-field gel electrophoresis (PFGE) was performed. Furthermore, 12 isolates of B. cereus were subjected to PFGE. Subsequently, whole-genome sequencing (WGS) analysis was conducted on ten of these isolates. The WGS data were analyzed and assembled using BioNumerics software. Multilocus sequence typing (MLST), virulence gene profiles, and single nucleotide polymorphisms (SNPs) were analyzed using assembled sequences.Results PFGE analysis classified the 12 B. cereus strains into nine pulsotypes. The three B. cereus isolates with the same PFGE pattern belonged to ST1435, and there were only three SNPs in the three ST1435 strains. The two B. cereus isolates with the same PFGE patterns were ST24 with one SNP between them, and the two ST24 isolates harbored hlbACD. These results indicate that the B. cereus isolates belonged to the same clone. The remaining three B. cereus strains also contained hlbACD.Conclusion Food-borne illness events caused by B. cereus are complex and are sources of contamination. Therefore, it will be necessary to strengthen the hygiene surveillance of food sources and workers and to pay more attention to cleaning and disinfecting environments and facilities, which will be important for preventing and controlling foodborne diseases.

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陈雯杰,陆冬磊,黄峥,顾其芳,毕磊,方沚昕,於颖,汪静,肖泉,张红芝.一起疑似由蜡样芽胞杆菌引起的食源性疾病事件的分子溯源研究[J].中国食品卫生杂志,2023,35(12):1799-1804.

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  • 收稿日期:2022-08-19
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  • 在线发布日期: 2024-04-03
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