超高效液相色谱-串联质谱法测定进口冷链食品外包装表面的三聚氰酸
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作者单位:

北京市疾病预防控制中心,食物中毒诊断溯源技术北京市重点实验室,北京 100013

作者简介:

姚凯 男 助理研究员 研究方向为食品中危害因子检测分析 E-mail:yaokaizz@163.com

通讯作者:

邵兵 男 研究员 研究方向为食品污染物分析 E-mail:shaobingch@sina.com

中图分类号:

R155

基金项目:

首都卫生发展科研专项(首发2022-4G-30118);北京市科技计划项目(Z211100007021008)


Determination of cyanuric acid in outer packaging of imported cold-chain food using ultra-high-performance liquid chromatography-tandem mass spectrometry
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Affiliation:

Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China

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    摘要:

    目的 建立用于测定进口冷链食品外包装表面的三聚氰酸的超高效液相色谱-串联质谱(UHPLC-MS/MS)分析方法。方法 以50%乙腈水溶液作为提取溶剂,对样品进行振荡提取,离心后取上层清液进样,采用Waters ACQUITY BEH HILIC(2.1×100 mm,1.7 μm)色谱柱进行分离,乙腈和10 mmol/L醋酸铵水溶液作为流动相等梯度洗脱,UHPLC-MS/MS检测三聚氰酸。结果 方法线性良好(相关系数>0.999 0),检出限和定量限分别为5.0~20.0和20.0~50.0 μg/kg,低、中和高添加水平下的平均回收率为80.0%~96.6%,日内和日间相对标准偏差均低于12.3%。结论 建立的方法前处理过程简便,回收率和灵敏度高且精密度良好,适用于进口冷链食品包装外表面三聚氰酸的检测。

    Abstract:

    Objective To develop an ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analytical method for the determination of cyanuric acid in the outer packaging of imported cold-chain food.Methods A 50% aqueous acetonitrile solution was used as an extraction solvent. The extraction was performed by shaking the samples. The sample was centrifuged, and the supernatant was transferred for injection. Cyanuric acid was separated using a Waters ACQUITY BEH HILIC (2.1×100 mm, 1.7 μm). The mobile phase consisted of acetonitrile and a 10 mmol/L aqueous ammonium acetate solution. An isocratic elution was also performed. Cyanuric acid was detected using UHPLC-MS/MS.Results Good linearity was achieved, with a correlation coefficient above 0.999 0. The limit of detection and limit of quantitation of the method were 5.0-20.0 and 20.0-50.0 μg/kg, respectively. The mean recoveries at low, middle, and high spiked levels were 80.0%-96.6%. The intra- and inter-day relative standard deviations were <12.3%.Conclusion Due to its straightforward sample preparation, reliable recovery, heightened sensitivity, and precision, the established method has been proven suitable for the assessment of cyanuric acid in the outer packaging of imported cold-chain food.

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姚凯,张晶,张昕,邵兵.超高效液相色谱-串联质谱法测定进口冷链食品外包装表面的三聚氰酸[J].中国食品卫生杂志,2023,35(12):1715-1721.

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  • 收稿日期:2023-01-06
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  • 在线发布日期: 2024-04-03
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