台州市售婴幼儿谷类辅助食品中克罗诺杆菌污染特征分析
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作者单位:

台州市疾病预防控制中心,浙江 台州 318000

作者简介:

陈喜凯 男 主管技师 研究方向为食品微生物学 E-mail:185chenkai888@163.con

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中图分类号:

R155

基金项目:

台州市科技局社会发展科技项目(20ywb106)


Analysis of Cronobacter contamination characteristics in cereal-based supplementary food for infants in Taizhou
Author:
Affiliation:

Taizhou Center for Disease Control and Prevention, Zhejiang Taizhou 318000, China

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    摘要:

    目的 了解台州市售婴幼儿谷类辅助食品中克罗诺杆菌的污染状况、分子分型特征和耐药性。方法 参照GB 4789.40—2016对样品进行定性和定量检测,分离到的菌株进行脉冲场凝胶电泳(PFGE)分型和微量肉汤稀释法药敏试验。结果 335份样品中分离到41株克罗诺杆菌,总检出率为12.2%;41份阳性样品定量结果为0.36~7.5 MPN/100 g;食用方式上烹煮类样品检出率最高(46.9%),购买途径上网购样品检出率最高(24.0%);41株菌株PFGE聚类分析被分为34个带型,相似度为45.1%~100.0%,从同一厂家不同种类样品和不同厂家样品中均有检出同一克隆株;菌株对头孢唑啉和呋喃妥因的敏感率仅为48.8%和39.0%,其中9株同时对这两种抗生素不敏感。结论 台州市售婴幼儿谷类辅助食品存在克罗诺杆菌的污染,应关注烹煮类和网购类食品导致婴幼儿感染克罗诺杆菌的风险。

    Abstract:

    Objective To investigate the contamination, molecular typing characteristics, and drug resistance of Cronobacter in cereal-based infant food in Taizhou.Methods All samples were detected qualitatively and quantitatively according to GB 4789.40—2016. Isolated strains were typed using pulse field gel electrophoresis (PFGE), and antibiotic susceptibility tests were performed using the micro-broth dilution method.Results Forty-one strains were isolated from 335 samples. The total detection rate was 12.2%, and the quantitative results were 0.36-7.5 MPN/100 g. The cooked sample (46.9%) had the highest detection rate among edible samples, and the sample purchased online (24.0%) had the highest detection rate among purchased samples. Forty-one strains were divided into 34 PFGE patterns with similarity rates of 45.1%-100.0%, and some strains from different enterprises or different branches of one enterprise belonged to the same clone. Their insensitivity rates to cefazolin and nitrofurantoin were 48.8% and 39.0%, respectively, and 9 strains were insensitive to both.Conclusion Some cereal-based supplementary foods for infants were contaminated with Cronobacter in Taizhou. It is necessary to pay attention to the risk of Cronobacter infection in infants caused by cooked food and food purchased online.

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陈喜凯,盛莹,沈伟伟,裘丹红.台州市售婴幼儿谷类辅助食品中克罗诺杆菌污染特征分析[J].中国食品卫生杂志,2023,35(7):1075-1079.

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  • 收稿日期:2022-08-15
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  • 在线发布日期: 2023-11-17
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