Objective A neuropsychosis poisoning caused by the ingestion of wild mushrooms in April 2021 in Lishui， Zhejiang Province， were investigated. The types of poisonous mushrooms were identified. Poisoning symptoms and treatment measures were reviewed.Methods Epidemiological investigation and clinical treatment analysis were performed. Field mushrooms were collected and speciated based on morphology and molecular biology.Results Epidemiological information revealed that six patients consumed mushrooms that were collected and used it for soup preparation. They developed poisoning symptoms approximately at 30 min to 2 h after consuming the soup. Mild symptoms included nausea， vomiting， and dizziness. Severe symptoms included diminished consciousness and involuntary limb twitching. Patients with mild symptoms recovered and were discharged from hospital on the same day following supportive treatment. Patients with severe symptoms recovered smoothly after hospitalization. Morphological and molecular analyses identified the mushrooms as Amanita pseudosychnopyramis.Conclusion Food poisoning caused by Amanita pseudosychnopyramis has not been previously reported.