2015—2020年宜宾市肉类食品沙门菌血清型及分子分型研究
作者:
作者单位:

1.成都医学院公共卫生学院,四川 成都 610500;2.宜宾市疾病预防控制中心,四川 宜宾 644600

作者简介:

刘欣玲 女 在读研究生 研究方向为卫生检验 E-mail:liuxinling202211@163.com

通讯作者:

张红 女 副主任技师 研究方向为微生物检验 E-mail:yb_zhanghong@126.com

中图分类号:

R155

基金项目:

国家自然科学基金(81601859);成都市科技局技术创新(2019-YF05-00104-SN);成都医学院-宜宾市疾病预防控制中心联合基金(2021LHYB05);省级大学生创新创业训练计划(S202013705080)


Salmonella serotyping and molecular typing in meat products in Yibin city of Sichuan Province from 2015 to 2020
Author:
Affiliation:

1.College of Public Health, Chengdu Medical College, Sichuan Chengdu 610500, China;2.Yibin Center for Disease Control and Prevention, Sichuan Yibin 644600, China

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    摘要:

    目的 研究宜宾市肉类食品中沙门菌分离株的血清型和分子分型特征,分析沙门菌污染的危险因素。方法 收集2015—2020年宜宾市辖区(县)肉类食品沙门菌分离株29株,进行血清型鉴定和脉冲场凝胶电泳(PFGE)分子分型分析,并就地域、物种来源和售卖方式对沙门菌血清群检出构成比的影响进行多因素Logistic回归分析。结果 29株沙门菌分离株共检出14种血清型(分属4个群),主要优势血清型为鼠伤寒沙门菌、吉韦沙门菌和肠炎沙门菌。聚类分析显示PFGE分子分型为18种带型。肠炎沙门菌带型相似度100%,来自同一传染链;吉韦沙门菌有2种带型,鼠伤寒沙门菌有4种带型,相似度较差;多因素Logistic分析显示,影响D群沙门菌检出的主要危险因素为地域分布(OR=1.053,P<0.05);影响E群沙门菌检出的主要危险因素为物种来源(OR=3.887,P<0.05)和地域分布(OR=1.227,P<0.05)。结论 监管部门应从家禽养殖地地域来源和肉类物种来源两个方面加强宜宾市肉类食品安全监督。

    Abstract:

    Objective To analyze the influencing factors of Salmonella contamination, the serotype distribution and molecular typing of Salmonella isolates in meat products in Yibin city were studied.Methods A total of 29 Salmonella strains isolated from commercial meat products in Yibin city of from 2015 to 2020 were analyzed by slide agglutination test for serotype and pulsed field gel electrophoresis(PFGE) for molecular subtyping. Multivariate Logistic regression analysis was carried out on the effects of geographical distribution, sample source and sales mode on the detection composition of Salmonella serogroup.Results Fourteen serotypes and 4 serogroups were detected in the Salmonella isolates. The dominant bacteria were Salmonella typhimuriumSalmonella give and Salmonella enteritidis. The PFGE cluster analysis showed 18 genotype. The genotypes of Salmonella enteritidis were highly homologous with similarity index of 100%, indicating the same source. Salmonella typhimurium with 4 genotypes and Salmonella give with 2 genotypes both had lower similarity. Multivariate Logistic analysis showed that the risk factor on serogroup D of Salmonella was geographical distribution (OR=1.053, P<0.05). The risk factors on serogroup E of Salmonella were sample source (OR=3.887, P<0.05) and geographical distribution (OR=1.227, P<0.05).Conclusion The poultry breeding sites and sample source are risk factors for Salmonella infection, and the hygienic inspection should be reinforced accordingly.

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刘欣玲,王烜,邓开旗,黄玥,张莹,李玖洪,毛俐,张红.2015—2020年宜宾市肉类食品沙门菌血清型及分子分型研究[J].中国食品卫生杂志,2023,35(3):321-325.

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  • 收稿日期:2022-01-22
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  • 在线发布日期: 2023-05-24
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