Objective To explore the feasibility of new test methods for evaluation of improving hypoxia tolerance function in health food.Methods Hypoxia tolerance experiments were carried out on mice. The zebrafish hypoxia model was established， and the improvement of zebrafish hypoxia movement and erythrocytosis were tested. A chemical hypoxia cell model was constructed with sodium disulfite， and cell activity and lactate dehydrogenase （LDH） activity were detected to verify their effect on hypoxia tolerance.Results Compared with normal control group， the hypoxia tolerance was improved in mice by health food sample. Compared with hypoxia model control groups， the sample improved zebrafish hypoxia， hypoxia-induced erycytosis， and alleviated the reduced cellular activity and increased LDH activity caused by cardiomyocyte hypoxia.Conclusion The ability of the sample to improve hypoxia tolerance can be detected by three test systems in vitro as well as in vivo， and the introduction of alternative methods to animal testing for hypoxia tolerance tests is feasible.