Association of Official Analytical Chemists standard for the determination of folate is more flexible， such as the use of strains， culture temperature， the use of more advanced equipment. There are still some gaps in these aspects in domestic standards. The relevant standards and regulations of the determination of folate in China， Codex Alimentarius Commission， European Union and the United States are compared from the aspects of the scope of application， strain， sample pretreatment， culture， determination method and others. In the process of drafting and revising the standards， the international standard system should be referenced and integrated gradually， standard review and training should be implemented to meet the needs of the lab.