Objective A high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of 42 kinds of antibiotics including quinolones， sulfonamides， nitroimidazoles and tetracyclines was developed for bean sprouts， dried bean curd and hot pot seasoning.Methods The samples were extracted with 70% acetonitrile-water （containing 0.1% formic acid）， purified by PRiME HLB， and separated by an Agilent Eclipse Plus C₁₈ column （150 mm×3.0 mm， 1.8 μm） with 0.2% formic acid acetonitrile and 0.2% formic acid water as gradient elution of mobile phase. The detection was performed under positive ion dynamic multiple reaction monitoring mode with electrospray ion source， and external standard method were used for quantification.Results The linear range of quinolones， sulfonamides and nitroimidazoles in bean sprouts， dried tofu and hot pot seasoning was 2-80 ng/mL， and the limit of detection was 5 μg/kg； the linear range of tetracyclines was 10-400 ng/mL， and the limit of detection was 25 μg/kg； the correlation coefficients were all greater than 0.997. The average recoveries of 42 kinds of antibiotics in three spiked levels were 85.4%-119.3%， and the relative standard deviations （n=6） was 0.4%-13.4%.Conclusion The method has a wide range of applications with easy operation and high sensitivity， and can rapidly screen the concentration of quinolones， sulfonamides， nitroimidazoles and tetracyclines in bean sprouts， dried tofu and hot pot seasoning.