Objective To develop a fast DNA release extraction reagent for foodborne pathogenic bacteria and optimize its application.Methods Orthogonal test was used to investigate the influence of Triton X-100（A）， SDS（B）， EDTA（C） and NP-40 （D）on the release effect. The influence of sample matrix on the release effect was determined by testing the actual sample with spiking standard， and the applicable process was further optimized.Results The optimal reagent combination was A1B2C2D2， which were Triton X-100 5.5%， SDS 0.04%， EDTA 2 mmol/L and NP-40 2%.Conclusion The reagent has a wide range of temperature adaptation with short time and high efficiency， which can be used for sample processing and nucleic acid extraction in poor experimental environment. It is convenient to use with low production cost and has application value for on-site quick inspection.