雅安市部分市售凉菜中金黄色葡萄球菌污染调查及分子分型
作者:
作者单位:

1.四川农业大学食品学院,食品加工与安全研究所,四川 雅安 625014;2.四川省食品检验研究院,四川 成都 611731

作者简介:

吴任之 男 微生物助理工程师 研究方向为食品药品微生物检测 E-mail:1414173602@qq.com

通讯作者:

韩国全 男 副教授 研究方向为微生物与食品安全及农产品加工与品质控制 E-mail:hans-980306@sicau.edu.cn

中图分类号:

R155

基金项目:


Investigation and molecular typing of Staphylococcus aureus in cold dishes sold in the market of Ya’an
Author:
Affiliation:

1.Sichuan Agricultural University Food College, Institute of Food Processing and Safety , Sichuan Ya’an 625014, China;2.Sichuan Food Inspection ReseArch Institute, Sichuan Chengdu 611731, China

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    摘要:

    目的 了解部分市售凉菜中金黄色葡萄球菌的污染状况、毒力基因分布、生物被膜形成能力及菌株分子分型特征。方法 从餐饮店、超市、流动摊点采集200份凉菜进行金黄色葡萄球菌分离鉴定,采用刚果红平板法及结晶紫染色法检测分离菌株生物被膜形成能力;通过PCR技术检测其毒力基因和生物被膜基因,并进行agr分型和多位点序列(MLST)分型。结果 共分离出24株金黄色葡萄球菌,检出率为10.50%(21/200);抽检的凉拌类样品检出率为13.73%(14/102),卤制类检出率为7.14%(7/98);传统肠毒素基因中seA检出率最高,为50.00%(12/24);而seE的检出率最低,为16.67%(4/24),中毒休克综合征毒素-1(TSST-1)与杀白细胞毒素(PVL)检出率分别为4.17%(1/24)和25.00%(6/24),溶血毒素基因检出率较高,脱皮毒素基因仅检出etd;刚果红平板法试验中阳性占62.5%(15/24),结晶紫染色法中阳性占100%;生物被膜基因以cnaclfB为主,未检出bap;菌株分子分型以agrⅠ和ST188为主。结论 部分市售凉菜中存在金黄色葡萄球菌污染,菌株携带多种毒力基因,成膜能力强,有潜在食品安全风险。

    Abstract:

    Objective To investigate the contamination status, virulence gene distribution, biofilm formation ability and molecular typing characteristics of Staphylococcus aureus S.aureus in some commercial cold dishes.Methods Two hundred cold dishes sold in restaurants, supermarkets and street vendor stalls were sampled, and Staphylococcus aureusS.aureus were separated. Congo red plate method and crystal violet staining method were used to detect the biofilmforming ability of the isolates. Virulence genes and biofilm genes were detected by PCR, as well as agr typing and multil-ocus sequence typing (MLST).Results Twenty four strains of S.aureus were isolated, and the detection rate was 10.50%(21/200); the detection rate of cold dishes was 13.73%(14/102), and the detection rate of stewed meat product was 7.14%(7/98); the highest detection rate of seA in the traditional enterotoxin gene was 50.00%(12/24), and the lowest of seE was 16.67%(4/24). Poisoning shock syndrome toxin-1 (TSST-1) and leukotoxin (PVL) were 4.17%(1/24) and 25.00%(6/24), respectively. The detection rate of hemolysin gene was relatively high, and only etd was detected in the dermatoxin gene. The positive rate was 62.5%(15/24) in Congo red plate test and 100% in crystal violet staining; the biofilm genes were mainly cna and clfB, and bap was not detected;the molecular typing of the strains was mainly agr Ⅰ and ST188.Conclusion The S.aureus contamination exsits in some cold dishes sold in Ya’an city. The strains carried multiple virulence genes, had strong film-forming ability and had potential food safety risks.

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吴任之,张翼,王鑫,代明涛,何小容,贺巧玲,韩国全.雅安市部分市售凉菜中金黄色葡萄球菌污染调查及分子分型[J].中国食品卫生杂志,2022,34(6):1257-1262.

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  • 收稿日期:2021-09-28
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  • 在线发布日期: 2023-02-06
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