基于酪胺信号放大技术的新型ELISA法高灵敏检测达氟沙星
作者:
作者单位:

1.南昌大学食品学院,江西 南昌 330029;2.江西省疾病预防控制中心/江西省食源性疾病诊断溯源重点实验室,江西 南昌 330029

作者简介:

鲍欢欢 女 硕士研究生 研究方向为食品质量与安全 Email: 2293575491@qq.com

通讯作者:

赖卫华 男 教授 研究方向为食品质量与安全 Email: talktolaiwh@163.com

中图分类号:

R155

基金项目:

国家重点研发计划(2019YFC1605502)


Development of novel ELISA based on tyramine signal amplification for sensitive detection of danofloxacin
Author:
Affiliation:

1.College of Food Science and Technology, Nanchang University, Jiangxi Nanchang 330029, China;2.Jiangxi Province Key Laboratory of Diagnosing and Tracing of Foodborne Disease, Jiangxi Province Centre for Disease Control and Prevention, Jiangxi Nanchang 330029, China

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    摘要:

    目的 建立一种结果可视的新型ELISA法高灵敏检测达氟沙星。方法 基于酪胺信号放大原理,设计一种高灵敏检测达氟沙星的新型ELISA法;通过灵敏度、特异性实验评估本方法的有效性;将本方法应用于鸡肉和猪肉加标样本的检测进行方法验证。结果 本方法裸眼检测灵敏度为0.4 ng/mL,相较于传统ELISA方法(10 ng/mL)的裸眼检测灵敏度提高了25倍;在缓冲液中与其他几种常用药物无明显交叉反应;对鸡肉和猪肉样本中加标的达氟沙星可有效检出。结论 本方法灵敏度高,特异性好,可用于实际样本中达氟沙星的现场裸眼定性检测。

    Abstract:

    Objective To develop a novel ELISA for sensitive and naked-eye detection of danofloxacin (DAN).Methods Based on TYR signal amplification, a novel ELISA was developed for sensitive detection of DAN. Analytical performance of the method was conducted by sensitivity and specificity experiments. The method was used for detecting DAN-spiked chicken and pork samples to verify.Results This method could be used to qualitatively detect DAN with naked eyes at a cut-off value of 0.4 ng/mL, which was 25-fold lower than that of the traditional ELISA (10 ng/mL). The method exhibited no cross-reaction of DAN with six other drugs in buffer. The method was successfully used for detecting DAN-spiked chicken and pork samples.Conclusion The developed novel ELISA exhibits high sensitivity and specificity, and can be used for the danofloxacin detection in actual samples by naked eye.

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鲍欢欢,刘成伟,刘道峰,赖卫华.基于酪胺信号放大技术的新型ELISA法高灵敏检测达氟沙星[J].中国食品卫生杂志,2022,34(3):517-523.

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  • 收稿日期:2022-01-28
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  • 在线发布日期: 2022-07-07
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