Objective To investigate the contamination, molecular typing characteristics and antibiotic resistance of Cronobacter in infants formula food in He'nan. Methods A total of 227 commercially available infants formula food were collected, and Cronobacter was isolated and identified according to GB 4789.40—2016. Pulse field gel electrophoresis (PFGE) and the broth microdilution method were used to confirm the molecular type and antibiotic resistance. 16S rRNA and multilocus sequence typing (MLST) were used to identify the species and analyze the phylogenetic evolution of Cronobacter. Results 13 Cronobacter strains isolated from 227 infants formula food were identified as C.sakazakii, C.turicensis and C.dublinensis. The antibiotic susceptibility result of 13 Cronobacter strains showed that the resistance rate of cefazolin reached 84.6% (11/13). 13 strains of Cronobacter were divided into 7 clusters and 6 ST types. Majority of the strains with the same PFGE type also had the same MLST type of which ST1 was the dominant type. Conclusion The main contamination of Cronobacter in infants formula food was caused by C.sakazakii with diverse biochemical phenotypes and genotypes. The molecular phylogenetic tree of MLST well interpreted the genetic relationship between different kind of species.