Objective Reference material candidates of bisphenol A, bisphenol S, bisphenol F and bisphenol AF in infant formula milk powders were prepared, in order to provide technical basis and methodology for the further formation of certified reference materials, and fill the research gap in this field. Methods The commercial infant formula milk powders were selected to prepare the reference material candidates by the processes of water dissolution, standard addition, homogenization, drying, crushing, screening and packaging. The concentrations of the target compounds were determined by isotope dilution-liquid chromatography tandem mass spectrometry. 25 bottles of samples were randomly selected for homogeneity study, and F test was used for statistical analysis. The long-term and short-term stability were studied by the quantitative analysis of samples stored at 25 ℃ for 12 months and at 60 ℃ for 7 d, respectively, and evaluated by univariate linear simulation equation. Results The homogeneity and stability result of the prepared reference material candidates were satisfactory, which met the expected requirements. Conclusion This work was expected to fill the gap in preparation of matrix reference materials for bisphenol A analogues, and provid material basis and technical support for the monitoring of bisphenol A analogues in food.