Objective To investigate the epidemiological characteristics and drug resistance of foodborne diseases in Jinnan District by monitoring and drug resistance analysis of foodborne diseases from 2018 to 2020, so as to provide basis for prevention and control of foodborne diseases. Methods The stool samples of 607 cases of foodborne diseases in sentinel hospitals of Jinnan District from 2018 to 2020 were detected for Salmonella, diarrheogenic Escherichia coli, Vibrio parahaemolyticus and Shigella, and the drug sensitivity of positive strains was analyzed. Results A total of 124 positive strains were detected in 607 stool samples, with a positive detection rate of 20.43% (124/607). There were 74 strains of Salmonella, 31 strains of Vibrio parahaemolyticus, 19 strains of diarrheogenic Escherichia coli, and no Shigella was detected. The ratio of male to female was 4∶3 (347/260). The highest detection rate in Salmonella was Salmonella enteritidis(62.16%, 46/74), and the highest detection rate in diarrheogenic Escherichia coli was enteropathogenic Escherichia coli (EPEC). In drug sensitivity test, Salmonella and diarrheogenic Escherichia coli were significantly resistant to ampicillin (AMP), up to 72.04% (67/93), and multiple drug resistance was common. Vibrio parahaemolyticus was most resistant to cefazolin (CFZ) (77.42%, 24/31). Conclusion Salmonella enteritidis and EPEC are the main pathogens of foodborne diseases in Jinnan District of Tianjin from 2018 to 2020. Strains are resistant to AMP with multi-drug resistance. It suggested that the relevant departments should strengthen the supervision of food processing industry, and strengthen the monitoring of drug resistance of pathogenic bacteria, so as to provide the basis for food safety and clinical treatment in Jinnan District.