2010—2018年江苏省淡水产品中副溶血性弧菌监测结果研究
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(江苏省疾病预防控制中心,江苏 南京 210009)

作者简介:

倪云龙 男 主管技师 主要研究方向为食品微生物检测与评估 E-mail:nylss2008@ 163.com

通讯作者:

霍翔 男 副主任医师 主要研究方向为流行病与卫生统计学 E-mail:huox@ foxmail.com

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Quantitative analysis of surveillance results the Vibrio parahaemolyticus in freshwater products in Jiangsu from 2010 to 2018
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(Jiangsu Provincial Center for Disease Control and Prevention, Jiangsu Nanjing 210009, China)

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    摘要:

    目的 掌握江苏省淡水产品中副溶血性弧菌污染水平和趋势,为风险评估提供基础定量数据。方法 2010—2018年在江苏省13个市的流通和餐饮环节的不同场所,采集8类淡水产品1 170份,按照GB 4789.7《食品安全国家标准 食品微生物学检验 副溶血性弧菌检验》进行副溶血性弧菌的定性和定量检验,使用@RISK软件对数据进行模拟分析,拟合定量污染数据。结果 1 170份淡水产品中副溶血性弧菌检出率28.0%,污染水平为(-1.01±1.58) lg MPN/g,毒力基因携带率3.7%。内陆地区副溶血性弧菌检出率和污染水平高于沿海地区,差异有统计学意义(P<0.05)。不同年份、不同地区、不同种类、不同月份和季度检出率和污染水平差异有统计学意义(P<0.05),不同采样场所检出率和污染水平差异无统计学意义(P>0.05)。内陆地区和沿海地区副溶血性弧菌污染水平分别为(-0.89±1.59) lg MPN/g和(-1.56±1.54) lg MPN/g;第二季度污染水平最高为(-0.56±1.72) lg MPN/g;饭店/酒店污染水平最高为(-0.68±1.55) lg MPN/g;淡水虾类中副溶血性弧菌污染水平最高为(-0.44±1.68) lg MPN/g。结论 江苏省淡水产品中副溶血性弧菌污染严重,副溶血性弧菌的污染由海产品向淡水产品转移,由沿海地区向内陆地区扩散,且有高于沿海地区的趋势。淡水产品可能是引起副溶血性弧菌感染的危险因素之一,淡水虾类中副溶血性弧菌污染严重,需对淡水产品开展风险评估。

    Abstract:

    Objective To investigate the contamination level and trend of Vibrio parahaemolyticus(VP) in freshwater products in Jiangsu Province, and provide basic quantitative data for the risk assessment. To grasp the contamination level and trend of Vibrio parahaemolyticus (VP) in freshwater products in Jiangsu Province, and provide basic quantitative data for the risk assessment. Methods One thousand one hundred and seventy samples form 8 categories freshwater products were collected from 2010 to 2018. The sampling sites include different locations of circulate and catering links in 13 cities of Jiangsu Province. The qualitative and quantitative data was obtained by method based on GB 4789.7 “Food microbiological examination-Examination of Vibrio parahemolyticus”. @RISK software was used to analyze the data and fitting quantitative contamination data. 1170 samples form 8 categories freshwater products were collected from 2010 to 2018. The sampling sites include different locations of circulate and catering links in 13 cities of Jiangsu Province. The qualitative and quantitative method based on GB 4789.7 “Food microbiological examination-Examination of Vibrio parahemolyticus”. Use @RISK software analyze the data and fitting quantitative contamination data.Results The detection rate of VP in 1 170 freshwater products was 28.0% and 3.7% VP carried virulence gene. The contamination levels of VP in freshwater products was (-1.01±1.57) lg MPN/g. The detection rate and contamination levels of VP in inland areas was higher than coastal areas, the difference was statistically significant (P<0.05). The difference of detection rates and contamination levels in different years, areas, categories, months and quarters were statistically significant (P<0.05). While the difference in different sampling sites was not statistically significant (P>0.05). The contamination levels of VP in inland areas and coastal areas were (-0.89±1.59) lg MPN/g and (-1.56±1.54) lg MPN/g. The contamination level in the second quarter, in the restaurant/hotel and freshwater shrimps were the highest, with the values of (-0.56±1.72) lg MPN/g, (-0.68±1.55) lg MPN/g and (-0.44±1.68) lg MPN/g, respectively. The detection rate of VP in 1170 freshwater products was 28.0% and 3.7% VP carrying virulence gene. The contamination levels of VP in freshwater products was -1.01±1.57 lg MPN/g. The detection rate and contamination levels of VP in inland areas was higher than coastal areas, the difference was statistically significant (P<0.05). The detection rates and contamination levels difference in different years, areas, categories, months and quarters were statistically significant (P<0.05). While the difference in different sampling sites was not statistically significant (P>0.05). The contamination levels of VP in inland areas and coastal areas were -0.89±1.59 lg MPN/g and -1.56±1.54 lg MPN/g. The contamination level in the second quarter, in the restaurant/hotel and freshwater shrimps were highest. The values were -0.56±1.72 lg MPN/g, -0.68±1.55 lg MPN/g and -0.44±1.68 lg MPN/g, respectively.Conclusion The contamination of VP in freshwater products in Jiangsu Province was seriously. The contamination of VP had shifted from seafood to freshwater products and spread from coastal areas to inland areas. Moreover, the contamination of inland area had a trend to exceed the coastal areas. Freshwater products might be the important risk factor for the disease of VP. The contamination of VP in freshwater shrimps were seriously. It is necessary to carry out the risk assessment for freshwater products. The contamination of VP in freshwater products in Jiangsu Province were seriously. The contamination of VP has shifted from seafood to freshwater products and spread from coastal areas to inland areas. Moreover, the contamination is a trend higher than coastal areas. Freshwater products maybe the important risk factor for the disease of VP. The contamination of VP in freshwater shrimps were seriously. It's necessary to carry out the risk assessment for freshwater products.

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倪云龙,乔昕,王燕梅,田亭,霍翔.2010—2018年江苏省淡水产品中副溶血性弧菌监测结果研究[J].中国食品卫生杂志,2021,33(4):455-462.

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  • 收稿日期:2021-03-10
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  • 在线发布日期: 2021-07-21
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