Objective To investigate the prevalence and drug resistance of Staphylococcus aureus in pig slaughterhouse pigs in He'nan Province. Methods Samples of nasal swabs and body surface swabs under different conditions, along with scalding water and air samples were collected, and were then cultured and inspected for Staphylococcus aureus isolation, through a procedure based on the national standard GB 4789.10-2016. Drug resistance of the staphylococcal isolates were tested by means of microbroth dilution method, following the antimicrobial susceptibility test standard of the American Institute of Clinical and Laboratory(CLSI) standards. Results The result showed that 96 strains of Staphylococcus aureus were isolated from 625 samples, accounting for 15.36%, among which the most positive ones were from samples of pigs to be slaughtered(33.00%,33/100), electronarcosis(25.83%,31/120) and decapitation(22.00%,22/100), while isolation rates were fairly low or even null in samples from depilation body surface, chilling body surface, split half body surface, air and water processes. The drug resistance result indicated that all the 96 Staphylococcal aureus strains presented resistance to the 8 kinds of test antimicrobials, especially florfenicol(98.96%,95/96). Almost all the strains (96.88%,93/96) showed multiple resistance to chloramphenicol, macrolides, lactams, lincoamines and other antimicrobials, and isolates resistant to all 7 kinds of tested drugs as accounted for 34.38% (33/96), and those resistant to 5 or plus drugs 76.04%(73/96). Conclusion There are different degrees of Staphylococcus aureus contamination in some slaughterhouses in He'nan Province, in which the pollution in waiting for slaughtering, hemp electricity and decapitation is more serious, which should be paid more attention. The overall drug resistance of Staphylococcus aureus isolates is serious, and there is a common phenomenon of multiple drug resistance, so the relevant departments need to strengthen the supervision of the use of antibiotics.