Objective To establish a method for determination of 6 kinds of carbamate pesticides and 3 kinds of chloronicotinyl pesticides in Chinese Kushui rose by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) coupled with QuEChERS. Methods After extracted by acetonitrile, the Chinese Kushui rose was cleaned by QuEChERS. The target compounds were separated by C18 column(2.1 mm×100 mm,1.7 μm) using 10 mmol/L ammonium acetate solution (0.1% formic acid) with acetonrtrile as mobile phase for gradient elution, and analyzed by MS/MS system with electrospray ionization(ESI+) under muti-reaction monitoring mode and quantified by external standard method. Results All the 9 kinds of pesticides showed good linear relationships in range of 0.01-0.50 μg/mL, and the correlation coefficients were above 0.990, the recoveries at different spiked levels for all target compounds in blank matrices were 76.3%-102%,and the relative standard deviation (RSD)were 1.3%-9.0%(n=6). The limits of detection and quantification of the method were 0.001 6-0.003 2 and 0.005 4-0.010 mg/kg. Conclusion The method was suitable for rapid screening and analysis of 9 pesticide residues in Chinese Kushui rose with the advantage of accuracy, rapidity, simplicity and high sensitivity.