Objective To investigate Salmonella contamination in food in Wenzhou, and to analyze the serotype distribution, drug resistance, and pulsed-field gel electophroresis(PFGE)patterns. Methods According to GB 4789.4-2016, the Salmonella strains were isolated, identified and serotyped. The broth microdilution method was used for drug sensitivity test. All strains were subtyped by PFGE. Results Six categories of 2 039 food samples were collected. 37 Salmonella strains were isolated with a positive isolation rate of 1.8%. Raw poultry meat and raw livestock meat got the highest detection rates with 6.9%(20/290) and 3.4%(10/290), respectively. The 37 Salmonella isolates belonged to 16 serotypes,including S. Typhimurium, S. Derby and S. Enteritidis as the dominant serotypes. 81.1%(30/37) of the strains showed different resistance levels to 17 kinds of antibiotics, and displayed 24 kinds of multiple drug resistant spectrums. The multiple antibiotic resistance rate reached 56.8%(21/37). The 37 isolates were divided into 31 PFGE patterns which showed polymorphism. Conclusion Food was contaminated to some degree by Salmonella in Wenzhou, and the antibiotic resistance was severe. The aggregation of PFGE patterns was related to the serotype of Salmonella, but had no obvious relation to the resistant spectrum.