Objective To investigate the characteristics of Clostridium perfringens(Cp) from 87 healthy people in Shunyi District of Beijing. To study the prevalence of cpe and the β2 toxin gene in the bacterial strain and the distribution characteristics of Cp. It will provide a baseline for the determination of foodborne diseases caused by Cp. Methods The stool of 87 health people were collected for Cp isolation culture, plate count and cpe and β2 genes test. Statistical analysis was performed on the test result. Results The detection rate of Cp in 87 stool samples was 64.37% (56/87). The detection rate showed no significant difference among different sex, age and month(P＞0.05). The result of 57 fecal specimens were above the detection limit(10 CFU/g), and the maximum quantitative value was 4.12×10⁶ CFU/g. The mean value was 1.70×10⁵ CFU/g, the median value was 5.30×10³ CFU/g and the 95% percentile value was 7.00×10⁵ CFU/g. The detection rate of cpe gene was 0.00%(0/56), and that of the β2 gene was 73.21% (41/56). Conclusion High Cp detection rate was found in 87 healthy people, the carrying rate of β2 gene was high. The foodborne diseases caused by Cp required more in-depth biological marker identification or quantitative analysis data to conduct research.