顺义区市售双壳贝类中副溶血性弧菌和诺如病毒的耐药性及分型研究
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(北京市顺义区疾病预防控制中心,北京 101300)

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王园园 女 主管检验师 研究方向为食品病原微生物检验 E-mail:415809126@qq.com

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Study on drug resistance and typing of Vibrio parahaemolyticus and Norovius in bivalve shellfish sold in Shunyi District
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(Shunyi District Center for Disease Control and Prevention, Beijing 101300,China)

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    摘要:

    目的 了解北京市顺义区部分市售双壳贝类中副溶血性弧菌及诺如病毒的污染情况,为相关感染性腹泻的防治提供依据。方法 2017年7~10月采集顺义区市场的双壳贝类,取适量样品分离其消化腺,进行样品前处理,提取病毒RNA后采用实时荧光定量逆转录-聚合酶链式反应(RT-PCR)法检测诺如病毒。剩余样品按照GB 4789.7—2013《食品安全国家标准 食品微生物学检验 副溶血性弧菌检验》进行副溶血性弧菌的分离鉴定。对检出的副溶血性弧菌进行毒力基因(tlh,tdh,trh)检测、耐药性分析和脉冲场凝胶电泳(PFGE)分子分型。结果 50份双壳贝类样品中有18份检出副溶血性弧菌(18株),检出率为36.0%;有8份样品携带诺如病毒。分离的副溶血性弧菌17株为tlh+tdh-trh-型,1株为tlh-tdh-trh-型;所有副溶血性弧菌均对氨苄西林耐药,对其他抗生素均敏感;PFGE图谱聚类后呈现多态性,存在多个克隆群。诺如病毒只在10月份检出,分布于牡蛎、蛏子和扇贝中,均为GⅡ型核酸阳性。结论 顺义区市售双壳贝类中存在副溶血性弧菌和GⅡ型诺如病毒,需警惕感染风险。分离的副溶血性弧菌呈现多态性,但毒力基因携带率较低,对大多数抗生素敏感。

    Abstract:

    Objective To study the pollution condition of Vibrio parahaemolyticus and Norovirus in some of bivalve mollusks sold in the market in Shunyi District and provide evidence for the prevention and treatment of infectious diarrhea. Methods The bivalve molluscs in the market of Shunyi District were collected from July to October of 2017. The appropriate samples were taken to separate the digestive glands, and virus RNA was extracted and real-time fluorescence quantitative reverse transcription-polymerase chain reaction(RT-PCR)was used to detect Noroviruses. The Vibrio parahaemolyticus was examined in the remaining samples following the national food safety standard GB 4789.7-2013.The genetic testing of virulence gene, drug resistance analysis and pulse field gel electrophoresis (PFGE) test were carried out on the isolated Vibrio parahaemolyticus. Results A total of 18 strains of Vibrio parahaemolyticus were detected among 50 samples of shellfish with the positive rate of 36.0%. Eight samples contained Norovirus. The virulence genetype of one isolate was tlh-tdh-trh- and the others were tlh+tdh-trh-. All isolates were resistant to ampicillin and sensitive to some other antibiotics. The PFGE patterns of different strains were polymorphic and belong to different clones. Norovirus was detected only in October and distributed in oysters, scorpions, and scallops, which are GⅡ-type nucleic acid positive. Conclusion The Vibrio parahaemolyticus and Norovirus of GⅡ were present in commercially available bivalve molluscs in Shunyi District. The isolated Vibrio parahaemolyticus was polymorphic, but the virulence genes had a low rate and were sensitive to most antibiotics.

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王园园,李颖,杨杰,王苗.顺义区市售双壳贝类中副溶血性弧菌和诺如病毒的耐药性及分型研究[J].中国食品卫生杂志,2018,30(4):420-424.

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  • 收稿日期:2018-05-18
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  • 在线发布日期: 2018-08-16
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