Objective To analyze the serotype and drug-resistance of Salmonella isolated from patients with infectious diarrhea in the sentinel hospital of Quanzhou City from 2013 to 2016,and provide references for the rational clinical medication. Methods Traditional glass agglutination and liquid suspension array were used for the serotyping of Salmonella isolated from patients with infectious diarrhea, and micro broth dilution method was used for the analysis of drug-resistance. Data was analyzed with Excel software. Results A total of 73 Salmonella strains were isolated from 460 specimens and divided into 11 serotypes, among which the prevalent serotypes were Salmonella saintpaul (42.47%,31/73), Salmonella arechavaleta (16.44%,12/73) and Salmonella blegdan (12.33%,9/73). For the traditional glass agglutination assay,61 serotypes (83.56%,61/73) were identified, with 12 undetermined, while for the liquid suspension array, 71 serotypes (97.26%,71/73) were identified, with only two undetermined. Analysis of the drug-resistance showed that all the isolated strains were resistant to cefazolin, cefotetan and tobramycin, 95.89%(70/73)to the amikacin, and 84.93%(62/73)to the gentamicin. In contrast, all the strains were sensitive to ertapenem, ciprofloxacin, levofloxacin and nitrofurantoin. Conclusion Clinical isolates of Salmonella from patients with infectious diarrhea in Quanzhou City from 2013 to 2016 were resistant to multiple antibiotics. Early identification of the serotypes and drug-resistance would contribute to the rational clinical medication