Objective To investigate Vibrio vulnificus contamination in seafood in Beijing region and compare the results between real time flourescence chain reaction (RT-PCR) and VITEK. Methods 105 samples of seafood were randomly collected from Beijing region and were detected by traditional method combined with molecular biology method. Comparison of accuracy between RT-PCR and VITEK identification method was also conducted. Results Among 105 seafood samples, there were 40 Vibrio vulnificus positive samples, and the positive ratio was 38.10%. The positive ratio for shrimp, shellfish and fisd were 52.38% (11/21), 37.88% (25/66) and 22.22%(4/18), respectively. Confirmed by rpoB gene sequencing, the accuracy was 100.00%(40/40) for RT-PCR, and 67.50%(27/40) for VITEK. Conclusion Vibrio vulnificus contamination in seafood was an existing problem in Beijing region. In order to prevent the occurrence of food safety events, an evaluation of the potential risk of Vibrio vulnificus contamination in seafood was in need.