Objective To investigate the levels of six indicator polychlorinated biphenyls (PCBs) in milk samples from some areas of Hubei Province. Methods Thirty locally milk samples were collected in 2014 from some areas of Hubei Province. Concentrations of six indicator PCBs in milk samples were measured by isotope dilution-high resolution gas chromatography-high resolution mass spectrometry (HRGC-HRMS). The daily intake of residents in Hubei Province through milk was calculated and compared with domestic and foreign studies, and the exposure risk was assessed. ResultsThe content of indicator PCBs in milk was 107.2-4 311.2 pg/g fat, the mean and median value was 699.9 and 433.9 pg/g fat, respectively. The compounds with the highest contribution rate in milk samples were PCB-28, followed by PCB-153 and PCB-138. The average and high level (P95) daily intake of residents in Hubei Province through milk is 0.001 47 and 0.005 25 ng/kg BW, respectively. Conclusion The concentrations of indicator PCBs in milk of Hubei were at low level and residents in this province had low health risks through milk intake.