Objective To understand the antibiotic resistance of foodborne Salmonella in Guangxi, so as to provide scientific evidence for the treatment of Salmonella infections. Methods Seventy strains of Salmonella from food and 234 from patients collected in 2016 in Guangxi were tested for susceptibility to 14 antibiotics of 8 groups by minimum inhibitory concentration(MIC). Resistance patterns were compared and analyzed. Results The Salmonella isolates from food and patients were all sensitive to imipenem (IPM) but resistant to the other 13 antibiotics different levels. The 70 strains from food showed the highest resistance to tetracycline(TET,45.71%,32/70). Among the 234 clinical isolates, resistance was most frequently observed in ampicillin (AMP,70.94%,166/234),followed by tetracycline (70.09%,164/234). The resistance to ampicillin, ampicillin/sulbactam (AMS), tetracycline, nalidixic acid (NAL), cefotaxime(CTX) and cefazolin (CFZ) was statistically different between food and clinical isolates (P<0.05). The Salmonella isolates of both sources showed broad antibiotic resistance spectrum. The common pattern was resistance to ACTT/S(ampicillin-chloramphenico l-tetracycline-trimethoprim/sulfamethoxazole). Conclusion The result indicated that the Salmonella strains isolated in Guangxi are highly resistant to a variety of drugs particularly penicillins, chloramphenicol, sulfonamides and tetracycline. This suggested the demand for enhanced monitoring and establishing scientific prevention and control strategy.