Abstract:Objective To understand the situation of mycotoxin residues in puerh tea from Guangzhou City, and provide reference basis for risk assessment and government supervision. Methods A total of 432 puerh tea samples were collected from wholesale markets, retail stores, super markets, catering units, online sellers and tea fair in Guangzhou City from 2013-2015. The concentrations of aflatoxin B1, B2, G1, G2 in the samples were determined by purification column high performance liquid chromatography (HPLC) with post column derivatization method. Nivalenol, deoxynivalenol and zearalenone were determined by high performance liquid chromatography-mass spectrometry (HPLC-MS). Results No aflatoxin B1, B2, G1, G2, nivalenol, deoxynivalenol and zearalenone in puerh tea samples could be detected by HPLC and HPLC-MS method. Conclusion There was no mycotoxin detected by HPLC and HPLC-MS national standard method. Puerh tea in the markets of Guangzhou City was not contaminated with mycotoxins.