Objective To determine the antibiotic susceptibility of isolated Salmonella spp. from import and export food and evaluate the spectrum of their multiple antimicrobial resistance,so as to provide the basis for food safety and rational clinical drug usage. Methods The Salmonella spp. of 65 isolated strains and 17 reference strains were tested for their antimicrobial susceptibility against 15 kinds of antibiotics by Kirby-Bauer method. The susceptibility was determined by the standard of Clinical and Laboratory Standards Institute (CLSI). Results All of the isolates were sensitive to cefepime, ceftriaxone, amikacin and cefotaxime. The highest drug resistance rate was for ampicillin (16.9%,11/65) and piperacillin (13.8%,9/65). Many isolates were resistant to tetracycline (13.8%,9/65), kanamycin (9.2%,6/65) and streptomycin (7.7%,5/65). Among 65 isolates,eleven strains (16.9%,11/65)were resistant to one antibiotic,one strain(1.5%,1/65) was resistant to two antibiotics, and 7(10.8%, 7/65) strains of multidrug resistance were detected which were resistant to three to six antibiotics. Conclusion Isolates of Salmonella spp. from import and export food in Beijing showed multiple drug resistance and the monitoring should be strengthened to ensure food safety.