Mycotoxin contamination in crops and food seriously affects human health, monitoring and prevention of mycotoxin is an important part of food safety assurance system. With the superiorities of rapidness, lable-free, high throughput and sensibility, surface plasmon resonance (SPR) has been widely used in drug screening, food testing, environment monitoring, clinical diagnosis and other fields. In this paper, the application of SPR biosensor in rapid screening of mycotoxins in food was reviewed.