To research the antagonizing effect of lycopene on alcohol-induced liver injury due to continuous ethanol intake in mice.Methods A total of 100 healthy adult of SPF male Kunming mice were randomly divided into 5 groups, including the control group, the model group, and the low, middle and high dose lycopene groups with different doses (0.33,0.67,2.00 g/L) of lycopene respectively by oral gavage at 0.2 ml/10 g once a day for 30 days, and the control group and model group were given corn oil. From the 31st day, 3 hours after administration, the control group was given distilled water, while the other 4 groups were given 56 degrees liquor by oral gavage at 0.12 ml/10 g once a day for 8 days. After the last administration, 5 groups were fasted for 16 h, then triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), protein carbonyl content in blood serum, and malonaldehyde (MDA), superoxide dismutase (SOD), glutathione perioxidase (GSH-Px) in live were tested, and mortality and cumulative survival time were calculated. Results Compared with the control group, the TG, ALT, AST, protein carbonyl and MDA level of the model group increased significantly (P<0.01). Compared with the model group, the mortality of middle and high dose groups was significantly reduced (P<0.05), and the cumulative survival time was extend. The SOD and GSH-Px levels were significantly higher (P<0.01), while the TG, ALT, AST, MDA and protein carbonyl content were obviously reduced (P<0.05, P<0.01). With the increasing lycopene doses, the TG, ALT, AST, MDA and protein carbonyl content were dropped, while the SOD and GSH-Px levels were on the rise.Conclusion Lycopene had a certain antagonizing effect on alcohol-induced liver injury in mice.