食源性沙门菌毒力岛基因分布及特征研究
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(1.白求恩国际和平医院传染肝病科,河北 石家庄 050082;2.石家庄市疾病预防控制中心微生物检验所,河北 石家庄 050011)

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牛莉娅 女 主治医师 研究方向为感染性疾病的临床诊治 E-mail:83040073@qq.com通信作者:┣┣(中)通信作者┫┫郭玉梅 女 主管技师 研究方向为病原菌分子分型与耐药 E-mail:guokexin2199@163.com

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Distribution and characteristics of the pathogenicity island of foodborne Salmonella
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(1.Department of Infectious and Liver disease, Bethune International Peace Hospital,Hebei Shijiazhuang 050082,China;2.Shijiazhuang Center for Disease Control and Prevention, Hebei Shijiazhuang 050011,China)

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    摘要:

    研究食源性沙门菌毒力岛的分布特征及其与食物中毒的关系。方法 收集市售食品、市售生鸡肉、食物中毒标本沙门菌分离株,进行血清学分型,并应用聚合酶链式反应方法检测沙门菌毒力岛(Salmonella pathogenicity island,SPI)基因SPI1~SPI5,进行统计学分析。结果 152株沙门菌菌株中,检出16种血清型,以肠炎沙门菌为优势血清型,不同来源的该血清型菌株的毒力岛谱型有差异。SPI1在食物中毒菌株中检出率最高,市售生鸡肉SPI1的携带率高于市售食品。结论 沙门菌的毒力岛谱型分布与菌株的不同来源相关,其中SPI1与沙门菌食物中毒呈正相关,市售生鸡肉有引起沙门菌食物中毒的潜在风险。

    Abstract:

    To study the distribution characteristics and correlation of the pathogenicity island of the foodborne Salmonella.Methods The Salmonella isolates were collected from commercial food, commercial raw chicken, and food poisoning samples. The serotype of Salmonella was determined by serology agglutinating method. SPI gene core protein genes SPI1-SPI5 of Salmonella were detected by polymerase chain reaction. The data were statistically analyzed. Results Among the 152 isolates, Salmonella enteritidis was the most common serotype. There were different pathogenicity island type spectrums from different sources. The highest rate of SPI1 was detected in food poisoning strains. SPI1 carrying rate of raw chicken isolates was significantly higher than other commercially foods.Conclusion Salmonella pathogenicity island type spectrum distribution related to the strain of different sources, pathogenicity island gene SPI1 was positively correlated with the food poisoning caused by Salmonella. There is a potential risk of food poisoning caused by raw chicken.

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牛莉娅,秦丽云,徐保红,王燕,杨新英,孙殿兴,郭玉梅.食源性沙门菌毒力岛基因分布及特征研究[J].中国食品卫生杂志,2017,29(2):131-135.

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  • 收稿日期:2017-02-04
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  • 在线发布日期: 2017-05-03
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