To establish a method for determination of microencapsulated β-carotene in compound vitamin tablets by reverse-phase high performance liquid chromatography.Methods Microcapsules in the samples were destructed with 0.1 mol/L sodium carbonate solution, and ultrasonically extracted with methanol-acetonitrile(7∶3,V/V)using Agilent SB-C₈ column. Methanol-acetonitrile(9∶1,V/V)was the mobile phase, and the detection wavelength was 448 nm. Results β-carotene was completely separated in the chromatographic conditions. The linear range was 0.41-6.20 μg/ml, the average recovery was 99.4%-103.3%, and the RSD was below 2.0%.Conclusion The method was fast and simple, the precision, recovery, and repeatability met the requirements, and could determine microencapsulated β-carotene in compound vitamin tablets.