To investigate the virulence gene, serotype and antibiotic resistance of Vibiro parahaemolyticus from different sources in Wuxi.Methods For 92 strains of Vibiro parahaemolyticus, PCR was used to detect virulence gene. Serotype was determined by serum agglutination test. Antibiotic resistance was performed by disc diffusion method. Results The detection rate of tdh gene among food poisoning strains was 85.7%(12/14). The detection rate of tdh among sentinel hospital strains was 88.6%(39/44). But the detection rate of tdh among environment strains was only 14.7%(5/34). Serotype O3 was found to be the dominant serotype among the strains from food poisoning(85.7%,12/14) and sentinel hospital(65.9%,29/44), while there was no dominant serotype found in environment strains. The rate of ampicillin resistance among food poisoning strains was 35.7%(5/14). The rate of ampicillin resistance among sentinel hospital strains was 65.9%(29/44)and was 11.8%(4/34)among environment strains. All strains were 100.0% sensitive to ampicillin/sulbactam, gentamycin, iminodiaeetic, thienamycin.Conclusion The virulence gene, serotype and antibiotic resistance of V.parahaemolyticus isolated from different sources were different. Ideal antibiotic for treatment of V.parahaemolyticus were ampicillin/sulbactam, gentamycin, iminodiaeetic and thienamycin.