To investigate the contamination situation of Staphylococcus aureus(SA) and the drug resistance of epidemic strains in animal food market of Nanning in 2015, and to provide data support for foodborne disease surveillance.Methods A total of 383 samples of different kinds of animal derived foods were collected and identified by national standard method. The drug resistance of 28 strains of Staphylococcus aureus was detected by K-B disk method, and the resistant gene was detected by PCR. Results The results of drug resistance test of 28 strains of Staphylococcus aureus isolated from 383 animal food samples showed that 10 of them were methicillin-resistant Staphylococcus aureus, twenty-eight strains were of different degree of antibiotic resistance, and four resistance genes were detected. Conclusion Staphylococcus aureus contamination in commercial animal foods poses a risk of food poisoning. The results of drug resistance test of isolates can provide reference for the food poisoning caused by Staphylococcus aureus in Nanning.