A method for determination of choline in infant formula by HPLC-ELSD was optimized.Methods The separation was operated on an analtical Agilent ZORBAX 300 SCX column(4.6 mm×250 mm,5 μm)with water (containing 30 mmol/L triethylamine):acetonitrile (80∶20, V/V) as mobile phase.Results The limit of detection was 4 μg/ml. The calibration curve was linear (r=0.998 1). The relative recoveries were 94.98%-100.9%, and relative standard deviations of intra-day and inter-day precision were 2.75% and 3.12%, respectively.Conclusion The method was simple and fast. Compared to the traditional method, analysis time was shorter, and the peak shape of choline is more narrow and symmetrical.