To investigate the influence of casein phosphopeptides and vitamin C combined on the bone density of rats.MethodsWistar male rats were randomly divided into 5 groups by body weight (three experimental groups and two control groups). Three experimental groups were low, medium and high dose groups with the dosage of 5,0, 30 times of human recommended amount respectively. All experimental groups were administered by gavage a low calcium foundation feed, and the efficient calcium capsule was administered once a day which contain casein phosphopeptides and vitamin C. Daily calcium carbonate consumption (as Ca) of calcium carbonate control group was the same as high dose group, and low calcium control group were administered an equal volume of deionized water by gavage. Bone density and other indicators were detected after three months. ResultsHigh dose of casein phosphopeptides and vitamin C could significantly increase the rat bone calcium content and femur midpoint of bone density, the high and medium dose could increase the femur distal end bone density, and all experimental groups' femur weight were no less than the calcium carbonate control group.ConclusionThe casein phosphopeptides and vitamin C combined could effectively increase bone mineral density